Gastric Urease

[Clinical studies on gastric urease and the serum-urease precipitation (by Freisinger)].

概 要 胃粘膜に おけ るureaseの 特異的存在 を検討 して,胃液ammoniaの 分泌反応 に伴 な う増 減や摘 出 胃粘腰ureaseのpH活 性 曲線(Nikoloff)上 の態度 な どを総合 して これ を確認 し,そ れが塩酸分泌 に対 す る粘膜 保護 の上に, ammonia産 生を通 して積極 的 な意義を有す るもの と認め た.さ らにFreisinger の最近発表せ る血清-urease間 の沈降反応 を追試 し,胃 潰瘍患者血清 におけ る同沈 降反応 力価 の増強 を 浮認 める とともに ,本 反応因子 を追究 して,こ れがγ-globulinに一致 して認め られるurease抗 体 とは異 る, α2-globulin内 のα2-macroglobulinを 主体 に演 じられ ることを明 らか に した.な お,胃 粘膜urease は...

Affinity purification of Helicobacter pylori urease. Relevance to gastric mucin adherence by urease protein.

A simple, reproducible and high yield method of Helicobacter pylori urease enzyme purification was developed using a heparinoid (Cellufine sulfate) affinity gel. The purification method involved two sequential steps using the same gel that takes advantage of the differential affinity of urease to the heparinoid at two levels of hydrogen ion concentration. SDS-polyacrylamide gel electrophoresis ...

The role of gastric urease in acid secretion.

The experiments described in this paper were undertaken to throw light on the possible role of gastric urease in the secretion of hydrochloric acid by the stomach. Mathews (1925) first suggested thatgastric hydrochloric acid might be produced by the reaction NJEI4Cl-NH3+HCl, and Luck (1924) showed that ammonia can be formed from urea by suspensions of ground dog gastric mucosa. Rigoni (1930), M...

The activity and function of gastric urease in the cat.

Compression of Histopathology, Culture and Rapid Urease Test in Diagnosis of Helicobacter Pylori in Gastric Biopsy Specimens

Abstract Background and Objective: Helicobacter pylori (H. Pylori) infection is related to chronic gastritis, peptic ulcer, duodenal ulcer and gastric cancer. Thus, identification and treatment of the infection have a considerable importance. The aim of this study was to compare three methods of Histopathology, Culture and Rapid Urease test (RUT) in identification of H. Pylori in gastric bio...